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🧬 Module I · Molecular Foundations
ATP
Adenosine Triphosphate
Energy currency of the cell; used in many biochemical reactions
ADP
Adenosine Diphosphate
Product of ATP hydrolysis; can be recycled back to ATP
AMP
Adenosine Monophosphate
Nucleotide involved in energy metabolism and signaling
CoA
Coenzyme A
Carrier of acyl groups in metabolic reactions
NAD⁺
Nicotinamide Adenine Dinucleotide
Electron carrier in redox reactions; accepts electrons to form NADH
NADH
Nicotinamide Adenine Dinucleotide (reduced)
Reduced form of NAD⁺; donates electrons to electron transport chain
FAD
Flavin Adenine Dinucleotide
Electron carrier; accepts electrons to form FADH₂
FADH₂
Flavin Adenine Dinucleotide (reduced)
Reduced form of FAD; donates electrons to electron transport chain
DNA
Deoxyribonucleic Acid
Genetic material that stores hereditary information
RNA
Ribonucleic Acid
Involved in protein synthesis and gene regulation
mRNA
Messenger RNA
Carries genetic code from DNA to ribosome
tRNA
Transfer RNA
Brings amino acids to ribosome during protein synthesis
rRNA
Ribosomal RNA
Structural and catalytic component of ribosomes
PAL
Phenylalanine Ammonia Lyase
Key enzyme in phenylpropanoid pathway
PPO
Polyphenol Oxidase
Enzyme responsible for browning in fruits and vegetables
PG
Polygalacturonase
Enzyme that breaks down pectin during fruit ripening
SDS
Safety Data Sheet
Document listing hazards and safety information for chemicals
PPE
Personal Protective Equipment
Safety gear including lab coat, goggles, gloves
⚡ Module II · Metabolism & Energy
RuBP
Ribulose-1,5-bisphosphate
CO₂ acceptor in Calvin cycle; 5-carbon molecule
3-PGA
3-Phosphoglycerate
First stable product of Calvin cycle (3-carbon)
G3P
Glyceraldehyde-3-phosphate
Three-carbon sugar produced in Calvin cycle
PSI
Photosystem I
Photosystem with reaction center P700; produces NADPH
PSII
Photosystem II
Photosystem with reaction center P680; splits water and produces O₂
P680
Pigment 680
Reaction center chlorophyll of PSII; absorbs 680 nm light
P700
Pigment 700
Reaction center chlorophyll of PSI; absorbs 700 nm light
PQ
Plastoquinone
Mobile electron carrier in photosynthetic electron transport chain
PC
Plastocyanin
Copper-containing protein that transfers electrons in photosynthesis
Cyt b₆f
Cytochrome b₆f complex
Protein complex that transfers electrons and pumps protons
Rubisco
Ribulose-1,5-bisphosphate carboxylase/oxygenase
Most abundant protein; fixes CO₂ in Calvin cycle
PEP
Phosphoenolpyruvate
Three-carbon molecule; CO₂ acceptor in C4 plants
PEPCase
PEP Carboxylase
Enzyme that fixes CO₂ in C4 plants
C3
C3 plants
Plants that fix CO₂ directly via Calvin cycle (first product 3-PGA)
C4
C4 plants
Plants that concentrate CO₂ using Kranz anatomy (first product oxaloacetate)
CAM
Crassulacean Acid Metabolism
Plants that fix CO₂ at night to conserve water
ETC
Electron Transport Chain
Series of protein complexes that transfer electrons and pump protons
ATP synthase
ATP synthase
Enzyme that produces ATP using proton gradient
TCA
Tricarboxylic Acid Cycle
Another name for Krebs cycle
Q₁₀
Temperature coefficient
Factor by which reaction rate increases for 10°C temperature rise
NR
Nitrate Reductase
Enzyme that reduces nitrate to nitrite
NiR
Nitrite Reductase
Enzyme that reduces nitrite to ammonium
GS
Glutamine Synthetase
Enzyme that incorporates ammonium into glutamine
GOGAT
Glutamate Synthase
Enzyme that transfers amino group to form glutamate
SOS
Salt Overly Sensitive
Pathway for salt tolerance (SOS1, SOS2, SOS3)
ACC
1-aminocyclopropane-1-carboxylic acid
Precursor to ethylene
ACS
ACC Synthase
Enzyme that produces ACC from SAM
ACO
ACC Oxidase
Enzyme that converts ACC to ethylene
SAM
S-adenosylmethionine
Methyl donor; precursor to ACC and ethylene
📈 Module III · Productivity & Quality
HI
Harvest Index
Ratio of economic yield to total biomass
LAI
Leaf Area Index
Total leaf area per ground area
RUE
Radiation Use Efficiency
Biomass produced per unit of intercepted light
°Bx
Brix
Measure of soluble solids (primarily sugars) in fruits
TA
Titratable Acidity
Measure of acid content in fruits
ORAC
Oxygen Radical Absorbance Capacity
Measure of antioxidant capacity
GAE
Gallic Acid Equivalents
Unit for measuring phenolic content
TE
Trolox Equivalents
Unit for antioxidant capacity measurement
🌵 Module IV · Stress & Environmental Biochemistry
ABA
Abscisic Acid
Stress hormone that promotes stomatal closure
JA
Jasmonic Acid
Hormone involved in defense against herbivores
MeJA
Methyl Jasmonate
Volatile form of jasmonic acid
ET
Ethylene
Gaseous hormone involved in ripening and stress
SA
Salicylic Acid
Hormone involved in pathogen defense
ROS
Reactive Oxygen Species
Partially reduced forms of oxygen (O₂⁻, H₂O₂, OH•)
SOD
Superoxide Dismutase
Antioxidant enzyme that converts O₂⁻ to H₂O₂
CAT
Catalase
Antioxidant enzyme that converts H₂O₂ to H₂O and O₂
APX
Ascorbate Peroxidase
Antioxidant enzyme that uses ascorbate to reduce H₂O₂
GR
Glutathione Reductase
Enzyme that regenerates reduced glutathione (GSH)
GSH
Reduced Glutathione
Tripeptide antioxidant; redox buffer
GSSG
Oxidized Glutathione
Oxidized form of glutathione (disulfide)
HSP
Heat Shock Protein
Molecular chaperones that refold denatured proteins
LEA
Late Embryogenesis Abundant
Proteins that protect cells during dehydration
CBF
C-repeat Binding Factor
Transcription factor in cold acclimation pathway
DREB
Dehydration Responsive Element Binding
Transcription factor in drought response
MDA
Malondialdehyde
Marker of lipid peroxidation and oxidative stress
RBOH
Respiratory Burst Oxidase Homolog
NADPH oxidase that produces superoxide
🔬 Module V · Analytical & Biochemical Techniques
HPLC
High Performance Liquid Chromatography
Chromatography technique using high pressure
GC
Gas Chromatography
Chromatography for volatile compounds
MS
Mass Spectrometry
Technique for identifying compounds by mass
GC-MS
Gas Chromatography-Mass Spectrometry
Combined technique for separation and identification
TLC
Thin Layer Chromatography
Chromatography on silica gel plates
Rf
Retention Factor
Distance moved by compound / distance moved by solvent front
UV-Vis
Ultraviolet-Visible
Type of spectrophotometry
A
Absorbance
Measure of light absorbed by a sample
ε
Molar Extinction Coefficient
Constant in Beer-Lambert law
BSA
Bovine Serum Albumin
Common protein standard in assays
U
International Unit
1 μmol product formed per minute
Km
Michaelis Constant
Substrate concentration at half Vmax
Vmax
Maximum Velocity
Maximum rate of enzyme-catalyzed reaction
SD
Standard Deviation
Measure of data spread around mean
SEM
Standard Error of the Mean
SD / √n; precision of mean estimate
ANOVA
Analysis of Variance
Statistical test for comparing multiple groups
p-value
Probability value
Probability of obtaining results by chance
R²
R-squared
Coefficient of determination; goodness of fit
🇪🇹 Module VI · Ethiopian Case Studies
CGA
Chlorogenic Acid
Major phenolic compound in coffee; antioxidant
STS
Silver Thiosulfate
Ethylene inhibitor used in cut flowers
1-MCP
1-Methylcyclopropene
Ethylene inhibitor (SmartFresh™)
MAP
Modified Atmosphere Packaging
Packaging that alters gas composition to extend shelf life
CA
Controlled Atmosphere
Storage with controlled O₂ and CO₂ levels
RH
Relative Humidity
Amount of water vapor in air relative to maximum
PWL
Physiological Weight Loss
Water loss from fresh produce during storage